High-Throughput Chemical Screening Identifies Compounds that Inhibit Different Stages of the Phytophthora agathidicida and Phytophthora cinnamomi Life Cycles.
Identifieur interne : 000983 ( Main/Exploration ); précédent : 000982; suivant : 000984High-Throughput Chemical Screening Identifies Compounds that Inhibit Different Stages of the Phytophthora agathidicida and Phytophthora cinnamomi Life Cycles.
Auteurs : Scott A. Lawrence [Nouvelle-Zélande] ; Charlotte B. Armstrong [Nouvelle-Zélande] ; Wayne M. Patrick [Nouvelle-Zélande] ; Monica L. Gerth [Nouvelle-Zélande]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2017.
Abstract
Oomycetes in the genus Phytophthora are among the most damaging plant pathogens worldwide. Two important species are Phytophthora cinnamomi, which causes root rot in thousands of native and agricultural plants, and Phytophthora agathidicida, which causes kauri dieback disease in New Zealand. As is the case for other Phytophthora species, management options for these two pathogens are limited. Here, we have screened over 100 compounds for their anti-oomycete activity, as a potential first step toward identifying new control strategies. Our screening identified eight compounds that showed activity against both Phytophthora species. These included five antibiotics, two copper compounds and a quaternary ammonium cation. These compounds were tested for their inhibitory action against three stages of the Phytophthora life cycle: mycelial growth, zoospore germination, and zoospore motility. The inhibitory effects of the compounds were broadly similar between the two Phytophthora species, but their effectiveness varied widely among life cycle stages. Mycelial growth was most successfully inhibited by the antibiotics chlortetracycline and paromomycin, and the quaternary ammonium salt benzethonium chloride. Copper chloride and copper sulfate were most effective at inhibiting zoospore germination and motility, whereas the five antibiotics showed relatively poor zoospore inhibition. Benzethonium chloride was identified as a promising antimicrobial, as it is effective across all three life cycle stages. While further testing is required to determine their efficacy and potential phytotoxicity in planta, we have provided new data on those agents that are, and those that are not, effective against P. agathidicida and P. cinnamomi. Additionally, we present here the first published protocol for producing zoospores from P. agathidicida, which will aid in the further study of this emerging pathogen.
DOI: 10.3389/fmicb.2017.01340
PubMed: 28769905
PubMed Central: PMC5515820
Affiliations:
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Lawrence</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Biochemistry, University of OtagoDunedin</wicri:regionArea><wicri:noRegion>University of OtagoDunedin</wicri:noRegion></affiliation></author><author><name sortKey="Armstrong, Charlotte B" sort="Armstrong, Charlotte B" uniqKey="Armstrong C" first="Charlotte B" last="Armstrong">Charlotte B. Armstrong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Biochemistry, University of OtagoDunedin</wicri:regionArea><wicri:noRegion>University of OtagoDunedin</wicri:noRegion></affiliation></author><author><name sortKey="Patrick, Wayne M" sort="Patrick, Wayne M" uniqKey="Patrick W" first="Wayne M" last="Patrick">Wayne M. Patrick</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Biochemistry, University of OtagoDunedin</wicri:regionArea><wicri:noRegion>University of OtagoDunedin</wicri:noRegion></affiliation></author><author><name sortKey="Gerth, Monica L" sort="Gerth, Monica L" uniqKey="Gerth M" first="Monica L" last="Gerth">Monica L. Gerth</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</nlm:affiliation><country xml:lang="fr">Nouvelle-Zélande</country><wicri:regionArea>Department of Biochemistry, University of OtagoDunedin</wicri:regionArea><wicri:noRegion>University of OtagoDunedin</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Oomycetes in the genus <i>Phytophthora</i> are among the most damaging plant pathogens worldwide. Two important species are <i>Phytophthora cinnamomi</i>, which causes root rot in thousands of native and agricultural plants, and <i>Phytophthora agathidicida</i>, which causes kauri dieback disease in New Zealand. As is the case for other <i>Phytophthora</i> species, management options for these two pathogens are limited. Here, we have screened over 100 compounds for their anti-oomycete activity, as a potential first step toward identifying new control strategies. Our screening identified eight compounds that showed activity against both <i>Phytophthora</i> species. These included five antibiotics, two copper compounds and a quaternary ammonium cation. These compounds were tested for their inhibitory action against three stages of the <i>Phytophthora</i> life cycle: mycelial growth, zoospore germination, and zoospore motility. The inhibitory effects of the compounds were broadly similar between the two <i>Phytophthora</i> species, but their effectiveness varied widely among life cycle stages. Mycelial growth was most successfully inhibited by the antibiotics chlortetracycline and paromomycin, and the quaternary ammonium salt benzethonium chloride. Copper chloride and copper sulfate were most effective at inhibiting zoospore germination and motility, whereas the five antibiotics showed relatively poor zoospore inhibition. Benzethonium chloride was identified as a promising antimicrobial, as it is effective across all three life cycle stages. While further testing is required to determine their efficacy and potential phytotoxicity <i>in planta</i>, we have provided new data on those agents that are, and those that are not, effective against <i>P. agathidicida</i> and <i>P. cinnamomi</i>. Additionally, we present here the first published protocol for producing zoospores from <i>P. agathidicida</i>, which will aid in the further study of this emerging pathogen.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28769905</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>High-Throughput Chemical Screening Identifies Compounds that Inhibit Different Stages of the <i>Phytophthora agathidicida</i> and <i>Phytophthora cinnamomi</i> Life Cycles.</ArticleTitle><Pagination><MedlinePgn>1340</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2017.01340</ELocationID><Abstract><AbstractText>Oomycetes in the genus <i>Phytophthora</i> are among the most damaging plant pathogens worldwide. Two important species are <i>Phytophthora cinnamomi</i>, which causes root rot in thousands of native and agricultural plants, and <i>Phytophthora agathidicida</i>, which causes kauri dieback disease in New Zealand. As is the case for other <i>Phytophthora</i> species, management options for these two pathogens are limited. Here, we have screened over 100 compounds for their anti-oomycete activity, as a potential first step toward identifying new control strategies. Our screening identified eight compounds that showed activity against both <i>Phytophthora</i> species. These included five antibiotics, two copper compounds and a quaternary ammonium cation. These compounds were tested for their inhibitory action against three stages of the <i>Phytophthora</i> life cycle: mycelial growth, zoospore germination, and zoospore motility. The inhibitory effects of the compounds were broadly similar between the two <i>Phytophthora</i> species, but their effectiveness varied widely among life cycle stages. Mycelial growth was most successfully inhibited by the antibiotics chlortetracycline and paromomycin, and the quaternary ammonium salt benzethonium chloride. Copper chloride and copper sulfate were most effective at inhibiting zoospore germination and motility, whereas the five antibiotics showed relatively poor zoospore inhibition. Benzethonium chloride was identified as a promising antimicrobial, as it is effective across all three life cycle stages. While further testing is required to determine their efficacy and potential phytotoxicity <i>in planta</i>, we have provided new data on those agents that are, and those that are not, effective against <i>P. agathidicida</i> and <i>P. cinnamomi</i>. Additionally, we present here the first published protocol for producing zoospores from <i>P. agathidicida</i>, which will aid in the further study of this emerging pathogen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lawrence</LastName><ForeName>Scott A</ForeName><Initials>SA</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Armstrong</LastName><ForeName>Charlotte B</ForeName><Initials>CB</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Patrick</LastName><ForeName>Wayne M</ForeName><Initials>WM</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerth</LastName><ForeName>Monica L</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Biochemistry, University of OtagoDunedin, New Zealand.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>07</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agathis australis</Keyword><Keyword MajorTopicYN="N">avocado root rot</Keyword><Keyword MajorTopicYN="N">high-throughput screening</Keyword><Keyword MajorTopicYN="N">kauri dieback</Keyword><Keyword MajorTopicYN="N">oomycetes</Keyword><Keyword MajorTopicYN="N">phenotype 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IdType="pubmed">15608699</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Nouvelle-Zélande</li></country></list><tree><country name="Nouvelle-Zélande"><noRegion><name sortKey="Lawrence, Scott A" sort="Lawrence, Scott A" uniqKey="Lawrence S" first="Scott A" last="Lawrence">Scott A. Lawrence</name></noRegion><name sortKey="Armstrong, Charlotte B" sort="Armstrong, Charlotte B" uniqKey="Armstrong C" first="Charlotte B" last="Armstrong">Charlotte B. Armstrong</name><name sortKey="Gerth, Monica L" sort="Gerth, Monica L" uniqKey="Gerth M" first="Monica L" last="Gerth">Monica L. Gerth</name><name sortKey="Patrick, Wayne M" sort="Patrick, Wayne M" uniqKey="Patrick W" first="Wayne M" last="Patrick">Wayne M. Patrick</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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